Initial intraorgan formation of mercapturic acid.

Intrahepatic conversion of a glutathione conjugate to its mercapturic acid. Metabolism of 1-chloro-2,4-dinitrobenzene in isolated perfused rat and guinea pig livers.

Because of the low hepatic activity of gamma-glutamyl-transferase in the rat, the liver is generally considered to play only a minor role in the degradation of glutathione conjugates, a limiting step in mercapturic acid formation. Recent findings indicate, however, that the liver has a prominent role in glutathione catabolism, particularly in species other than rat. To examine the contributions...

Formation of Mercapturic Acids

& Young (1959, 1960) showed that n-propylmercapturic acid was excreted by rats, rabbits, guinea pigs and mice to which 1-bromoor 1-iodopropane had been administered. Bray & James (1958) obtained evidence suggesting that 1-bromobutane, -pentane, -hexane, -heptane and -octane, and 1-nitro-ethane and -propane, formed mercapturic acids in the rabbit, and Barnes, James & Wood (1959) showed that a si...

Excretion of the mercapturic acid S-[2-(N7-guanyl)ethyl]-N-acetylcysteine in urine following administration of ethylene dibromide to rats.

Administration of the carcinogen ethylene dibromide (EDB) to rats resulted in the urinary excretion of S-[2-(N7-guanyl)ethyl]-N-acetylcysteine, which is derived from the nucleic acid adduct S-[2-(N7-guanyl)ethyl]glutathione. This mercapturic acid was isolated from urine by reversed-phase and propylamino high-performance liquid chromatography and was quantitated by measurement of fluorescence in...

The formation of mercapturic acids. 1. Formation of mercapturic acid and the levels of glutathione in tissues.

The metabolism of 2:4-,2:5-and 3:4-dichloronitrobenzene in the rabbit.

an appreciable proportion of a dose of most of these compounds was converted into mercapturic acid in the rabbit. These mercapturic acids had been formed by a process resulting in the substitution of either a nitro or a chloro group by the acetylcysteyl group (acetylcysteyldenitration or acetylcysteyldechlorination) instead of replacement of a nuclear hydrogen as had been observed in all previo...